Skip to main content
Figure 1 | Malaria Journal

Figure 1

From: A kinetic fluorescence assay reveals unusual features of Ca++ uptake in Plasmodium falciparum-infected erythrocytes

Figure 1

Kinetics of fluorescence development. (A) Fluorescence kinetics in 96 well format. Symbols represent means ± S.E.M. of replicate wells for Dd2-infected and uninfected cells (blue and red symbols, respectively) in 1 mM free external Ca++ or 1 mM EGTA (circles and triangles, respectively). Notice greater fluorescence with Ca++ than EGTA for both cell types. A difference between infected and uninfected cells is apparent. (B) Identical experiment using 384 well format. While fluorescence development is specific for Ca++, the greater permeability of infected cells is not clearly resolved in this format. (C) Mean ± S.E.M. fluorescence after 12 hours with 1 mM free Ca++ for Dd2-infected and uninfected cells (blue and red bars, respectively) in indicated formats. n =4-8 independent trials each; P = 0.01 and 0.7 for comparisons of infected vs. uninfected cells in 96- and 384-well formats, respectively.

Back to article page