Figure 6
Conserved parasite requirement for extracellular Ca++. (A) Normalized growth of divergent parasite lines over 72 hours as a function of measured free external [Ca++], achieved by EGTA addition to standard media. Absissca is shown on log scale. Symbols represent mean ± S.E.M. growth of HB3, 3D7A, and Dd2 lines (green, white, and blue circles, respectively). Solid line represents the best fit to a sigmoidal decay. (B) Mean ± S.E.M normalized growth of each line when 2 mM CaCl2 is added to the medium with 2 mM EGTA, the highest used in panel (A).