Table 1 Specific primers detecting variant SSU rDNA sequences in 4 DNA samples
Reaction turn | Primer sequences | Nucleotide position | Product size |
|---|---|---|---|
First round | Â | Â | Â |
PoWu | 5′-CGTTTCTGAGGTGCTACGCTTGG-3′ | R2 (Low similarity) | 910 bp |
PoWL1 | 5′-GACGACACTCGACTCGGTTATCC-3′ | R7(Low similarity) | |
Second round | Â | Â | Â |
PoWu | 5′-CGTTTCTGAGGTGCTACGCTTGG-3′ | R2(Low similarity) | 440 bp |
PoWL2 | 5′-GTATCTGATCGTCTTCACTCCCTT-3′ | R4(High similarity) |