Figure 7

Absence of fibrosis in ALI/ARDS lungs. Light microscopy of Masson’s Trichrome stain (A and D), Picrosirius Red (B and E) and TEM (C and F) of non-infected control (A, B and C) and ALI/ARDS (D, E and F) lungs. Masson’s Trichrome technique stains collagen blue of the non-infected lung (A). There was no evidence of fibrosis in the ALI/ARDS lungs. Collagen was visible in small, localized areas by TEM in both the non-infected and ALI/ARDS lungs (B and D). ALI/ARDS lung, lung from DBA/2 mouse that died with acute lung injury/acute respiratory distress syndrome after infection with P. berghei ANKA. Arrow heads, localized areas of collagen. Note image (B) is a close up of tissue shown in 2B.