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Figure 2 | Malaria Journal

Figure 2

From: The development of loop-mediated isothermal amplification targeting alpha-tubulin DNA for the rapid detection of Plasmodium vivax

Figure 2

Sensitivity and specificity of the P. vivax α-tubulin LAMP assay. (A) Sensitivity of the P. vivax α-tubulin LAMP assay. Serial 10-fold dilutions of the α-tubulin DNA (10, 102, 103, and 104 copies per reaction) were used for the LAMP assay, and the real-time amplification was monitored by a measurement of absorbance. (B) Correlation between the threshold time and the copy number of the α-tubulin DNA in serially diluted samples. The values on the y-axis are the threshold time (in min), which was defined as the time at which the threshold value of absorbance (0.1) was reached. The results show the means and standard deviations of three separate experiments. A plasmid containing no insert was used as a control. The LAMP products were visualized using (C) gel electrophoresis and (D) Loopamp® fluorescent detection reagent (FD). Lane M, a 100-bp molecular weight marker; lane 1, P. falciparum; lane 2, Plasmodium ovale curtisi; lane 3, Plasmodium ovale wallikeri; lane 4, Plasmodium malariae; lane 5, Plasmodium knowlesi; lane 6, P. vivax; lane 7, Babesia microti; lane 8, Toxoplasma gondii; lane 9, Cryptosporidium parvum; lane 10, Entamoeba histolytica; lane 11, Giardia lamblia; lane 12, Trichomonas vaginalis; lane 13; Acanthamoeba castellanii; lane 14, malaria-negative human DNA control; lane R, products of the AatII digestion of the LAMP product of α-tubulin.

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