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Figure 1 | Malaria Journal

Figure 1

From: Performance of coumarin-derived dendrimer-based fluorescence-linked immunosorbent assay (FLISA) to detect malaria antigen

Figure 1

Schematic illustration of FLISA with novel monoclonal antibodies targeting pLDH and coumarin-derived dendrimer. LED-based FLISA consists of a capture antibody, analytes and coumarin-derived dendrimer conjugated mAbs. Plate was coated with capture antibody for overnight. Meanwhile, amine group of mAbs react with N-hydroxysuccinimide (NHS) of coumarin-derived dendrimer buffer (pH 8.5) to form stable amide bonds upon release of NHS. After washing, LDHs from analytes were detected by coumarin-derived dendrimer conjugated antibody and LED induced excitation of coumarin-derived dendrimer at 460 nm and emission at 590 nm were detected.

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