Figure 3

CHI3L1 increases during experimental cerebral malaria but does not affect survival. (A) Plasma was collected over a time course for P. berghei ANKA infection of C57BL/6 mice, and assayed for CHI3L1 by ELISA. **p < 0.01, ***p < 0.001 by Kruskal-Wallis test with Dunn’s post-test. (B) On Days 0 and 5 of infection, Chi3l1 transcripts in brain were measured by quantitative real-time PCR and normalized to housekeeping genes. *p < 0.05 by Mann–Whitney U test. (C) Four independent experiments were pooled to generate the survival curves shown (Chi3l1^−/−, n = 65; Wild-type littermates (WT), n = 51). The log-rank test was used to assess survival differences (p = 0.13). (D) Parasitaemia is represented as medians and interquartile range. (E) Plasma was collected from wild-type and Chi3l1^−/− mice on Day 4 of infection and assayed for cytokines. NS, not significant, Mann Whitney U test. Cytokines were undetectable in all uninfected mice.