Table 3 Suggested primer sequences, PCR conditions and expected product sizes[19, 22]

Pf3D7_0831800

5′CAA AAG GAC TTA ATT TAA ATA AGA G3′(forward)

55°C

600 -1000 bp

Pfhrp2 exon 2

5′ AAT AAA TTT AAT GGC GTA GGC A3′(reverse)

5′ ATT ATT ACA CGA AAC TCA AGC AC3′(nested forward)

Pf3D7_0831800

5′ TAT CCG CTG CCG TTT TTG CC 3′

57°C

303 bp

Pfhrp2 exon 1-2

5′ AGC ATG ATG GGC ATC ATC CTA 3′

Pf3D7_0831700

5′ AGA CAA GCT ACC AAA GAT GCA GGT G 3′

60°C

227 bp

(MAL7P1_228)

5′ TAA ATG TGT ATC TCC TGA GGT AGC 3′

Pf3D7_0831900

5′ TAT GAA CGC AAT TTA AGT GAG GCA G 3′

68°C

346 bp

(MAL7P1_230)

5′ TAT CCA ATC CTT CCT TTG CAA CAC C 3′