Table 2 Sensitivity and specificity of the PET-PCR assay and microscopy when compared to nested 18S rRNA PCR
Reference standard | |||
|---|---|---|---|
Test result | Nested 18S rRNA PCR | ||
Multiplex PET PCR | Present (+) | Absent (−) | |
Test positive (+) | 27 | 0 | 27 (18.75%) |
Test negative (−) | 0 | 117 | 117 (81.25%) |
27 (18.75%) | 117 (81.25%) | 144 (100.00%) | |
95% confidence interval: | |||
Lower limit | Upper limit | ||
Sensitivity | 100.00% | 94.04% | 100.00% |
Specificity | 100.00% | 96.10% | 100.00% |
PPV | 100.00% | 100.00% | 100.00% |
NPV | 100.00% | 100.00% | 100.00% |
Reference standard: | |||
Test result: | Nested 18S rRNA PCR | ||
Microscopy | Present (+) | Absent (−) | |
Test positive (+) | 11 | 0 | 11 (7.63%) |
Test negative (−) | 16 | 117 | 133 (92.36%) |
27 (18.75%) | 117 (81.25%) | 144 (100.00%) | |
95% confidence interval: | |||
Lower limit | Upper limit | ||
Sensitivity | 40.70% | 23.00% | 61.00% |
Specificity | 100.00% | 96.00% | 100.00% |
PPV | 100.00% | 67.90% | 100.00% |
NPV | 87.70% | 80.90% | 92.80% |