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Figure 5 | Malaria Journal

Figure 5

From: A novel approach for the discovery of chemically diverse anti-malarial compounds targeting the Plasmodium falciparum Coenzyme A synthesis pathway

Figure 5

Re-evaluation of higher potency compounds near individual IC 80 . A: Compounds that did not confirm at 2 μM concentration underwent additional testing at a lower concentration near their individual IC80 values. Direct comparison of compound activity with (black) and without (grey) addition of CoA is shown. At the indicated concentrations, three compounds were rescued to levels of ≥80% (top row) and two to approximately 50% of untreated control values (bottom row). Dashed lines indicate 100% of untreated control values, bars reaching values over 100% are shown as 100% . The bar graphs for original hits show n = 2, duplicate point for hits originating from the focussed compound library and n = 1, single point for compounds of the MMV malaria box. Re-test bar graphs show n = 1; duplicate point each; error bars indicate +/- standard error of mean (SEM). B: Compound 5964083 was the only compound that failed to reconfirm both at 2 μM and at a concentration near its IC80 value. The bar graph of the original hit depicts n = 2, duplicate point. All re-test bar graphs show n = 1; duplicate point each; error bars indicate +/- standard error of mean (SEM).

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