Table 1 Size, RFLP pattern and allelic frequency of Pvmsp - 3α gene
Size variation | RFLP by Alu 1 | Alleles | Data analysis n (%) frequency distribution of sub-allelic variants | ||||
|---|---|---|---|---|---|---|---|
Punjab | Sindh | Khyber Pakhtunkhwa | Balochistan | Total | |||
(n = 32) | (n = 27) | (n = 41) | (n = 30) | (n = 130) | |||
A | A1—A12 | 1 | 2 (6.25) | 2 (7.40) | 2 (4.87) | 2 (6.66) | 8 (6.15) |
2 | 2 (6.25) | 1 (3.70) | 2 (4.87) | 1 (3.33) | 6 (4.61) | ||
3 | 3 (9.37) | 2 (7.40) | 4 (9.75) | 2 (6.66) | 11 (8.46) | ||
4 | 2 (6.25) | 1 (3.70) | 2 (4.87) | 1 (3.33) | 6 (4.61) | ||
5 | 1 (3.12) | 1 (3.70) | 1 (2.43) | 1 (3.33) | 4 (3.07) | ||
6 | 2 (6.25) | 1 (3.70) | 1 (2.43) | 1 (3.33) | 4 (3.07) | ||
7 | 0 (0.00) | 1 (3.70) | 1 (2.43) | 1 (3.33) | 3 (2.30) | ||
8 | 1 (3.12) | 0 (0.00) | 1 (2.43) | 1 (3.33) | 3 (2.30) | ||
9 | 3 (9.37) | 3 (11.11) | 4 (9.75) | 3 (10) | 13 (10) | ||
10 | 0 (0.00) | 0 (0.00) | 1 (2.43) | 1 (3.33) | 2 (1.53) | ||
11 | 2 (6.25) | 0 (0.00) | 1 (2.43) | 1 (3.33) | 3 (2.30) | ||
12 | 0 (0.00) | 0 (0.00) | 1 (2.43) | 0 (0.00) | 1 (0.76) | ||
B | B1—B5 | 13 | 3 (9.37) | 3 (11.11) | 3 (7.31) | 2 (6.66) | 11 (8.46) |
14 | 2 (6.25) | 3 (11.11) | 3 (7.31) | 3 (10) | 11(8.46) | ||
15 | 1 (3.12) | 1 (3.70) | 1 (2.43) | 1 (3.33) | 4 (3.07) | ||
16 | 0 (0.00) | 0 (0.00) | 1 (2.43) | 0 (0.00) | 1 (0.76) | ||
17 | 0 (0.00) | 0 (0.00) | 0 (0.00) | 0 (0.00) | 0 (0.00) | ||
C | C1—C4 | 18 | 2 (6.25) | 1 (3.70) | 2 (4.87) | 1 (3.33) | 6 (4.61) |
19 | 4 (12.5) | 3 (11.11) | 4 (9.75) | 4 (13.33) | 15 (11.53) | ||
20 | 2 (6.25) | 1 (3.70) | 2 (4.87) | 1 (3.33) | 6 (4.61) | ||
21 | 0 (0.00) | 0 (0.00) | 0 (0.00) | 0 (0.00) | 0 (0.00) | ||
D | D1 | 22 | 2 (6.25) | 2 (7.40) | 3 (7.31) | 2 (6.66) | 9 (6.92) |
M | M1 | 23 | 0 (0.00) | 1 (3.70) | 1 (2.43) | 1 (3.33) | 3 (2.30) |
Total frequency (%) | 32 (24.61) | 27 (20.76) | 41 (31.53) | 30 (23.07) | 130 (64.35) | ||
Mean ± SE | 1.48 ± 0.80 | 1.30 ± 0.96 | 1.17 ± 0.92 | 1.78 ± 0.79 | |||
Kruskal wallis test | p < 0.05 | ||||||