Table 2 Size, RFLP pattern and allelic frequency of Pvmsp-3β gene
Size variation | RFLP byPst1 | Alleles | Data analysis n (%) frequency distribution of sub-allelic variants | ||||
|---|---|---|---|---|---|---|---|
Punjab | Sindh | Khyber Pakhtunkhwa | Balochistan | Total | |||
(n = 21) | (n = 20) | (n = 17) | (n = 14) | (n = 72) | |||
A | A1—A7 | A1 | 1(4.76) | 1 (5) | 0 (0.00) | 1 (7.14) | 3 (4.16) |
A2 | 1(4.76) | 1 (5) | 0 (0.00) | 0 (0.00) | 2 (2.77) | ||
A3 | 1(4.76) | 1 (5) | 1 (5.88) | 0 (0.00) | 3 (4.16) | ||
A4 | 1(4.76) | 2 (10) | 1 (5.88) | 1 (7.14) | 5 (6.94) | ||
A5 | 1(4.76) | 0 (0.00) | 1 (5.88) | 0 (0.00) | 2 (2.77) | ||
A6 | 0 (0.00) | 0 (0.00) | 1 (5.88) | 0 (0.00) | 1 (1.38) | ||
A7 | - | - | - | - | - | ||
B | B1—B10 | B1 | 1(4.76) | 1 (5) | 1 (5.88) | 1 (7.14) | 4 (5.55) |
B2 | 2 (9.52) | 2 (10) | 2 (11.76) | 2 (14.28) | 8 (11.11) | ||
B3 | 2 (9.52) | 2 (10) | 0 (0.00) | 1 (7.14) | 5 (6.94) | ||
B4 | 1(4.76) | 1 (5) | 1 (5.88) | 0 (0.00) | 3 (4.16) | ||
B5 | 1(4.76) | 1 (5) | 1 (5.88) | 1 (7.14) | 4 (5.55) | ||
B6 | 2 (9.52) | 3 (15) | 2 (11.76) | 0 (0.00) | 7 (9.72) | ||
B7 | 0 (0.00) | 0 (0.00) | 0 (0.00) | 1 (7.14) | 1 (1.38) | ||
B8 | - | - | - | - | - | ||
B9 | - | - | - | - | - | ||
B10 | - | - | - | - | - | ||
C | C1—C4 | C1 | 2 (9.52) | 2 (10) | 2 (11.76) | 1 (7.14) | 7 (9.72) |
C2 | 2 (9.52) | 1 (5) | 1 (5.88) | 1 (7.14) | 5 (6.94) | ||
C3 | 1(4.76) | 1 (5) | 0 (0.00) | 1 (7.14) | 3 (4.16) | ||
C4 | 2 (9.52) | 1 (5) | 1 (5.88) | 1 (7.14) | 5 (6.94) | ||
C5 | 0 (0.00) | 0 (0.00) | 1 (5.88) | 1 (7.14) | 2 (2.77) | ||
C6 | - | - | - | - | - | ||
M | M1 | M1 | 0 (0.00) | 0 (0.00) | 1 (5.88) | 1 (7.14) | 2 (2.77) |
Total frequency (%) | 21 (29.16) | 20 (27.77) | 17 (23.61) | 14 (19.44) | 72 (35.64) | ||
Mean ± SE | 1.11 ± 0.17 | 1.05 ± 0.19 | 0.89 ± 0.15 | 0.74 ± 0.13 | |||
Kruskal wallis test | p < 0.05 | ||||||