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Table 2 Size, RFLP pattern and allelic frequency of Pvmsp-3β gene

From: PCR/RFLP-Based Analysis of Genetically Distinct Plasmodium vivax Population of Pvmsp-3α and Pvmsp-3β genes in Pakistan

Size variation RFLP byPst1 Alleles Data analysis n (%) frequency distribution of sub-allelic variants
    Punjab Sindh Khyber Pakhtunkhwa Balochistan Total
    (n = 21) (n = 20) (n = 17) (n = 14) (n = 72)
A A1—A7 A1 1(4.76) 1 (5) 0 (0.00) 1 (7.14) 3 (4.16)
   A2 1(4.76) 1 (5) 0 (0.00) 0 (0.00) 2 (2.77)
   A3 1(4.76) 1 (5) 1 (5.88) 0 (0.00) 3 (4.16)
   A4 1(4.76) 2 (10) 1 (5.88) 1 (7.14) 5 (6.94)
   A5 1(4.76) 0 (0.00) 1 (5.88) 0 (0.00) 2 (2.77)
   A6 0 (0.00) 0 (0.00) 1 (5.88) 0 (0.00) 1 (1.38)
   A7 - - - - -
B B1—B10 B1 1(4.76) 1 (5) 1 (5.88) 1 (7.14) 4 (5.55)
   B2 2 (9.52) 2 (10) 2 (11.76) 2 (14.28) 8 (11.11)
   B3 2 (9.52) 2 (10) 0 (0.00) 1 (7.14) 5 (6.94)
   B4 1(4.76) 1 (5) 1 (5.88) 0 (0.00) 3 (4.16)
   B5 1(4.76) 1 (5) 1 (5.88) 1 (7.14) 4 (5.55)
   B6 2 (9.52) 3 (15) 2 (11.76) 0 (0.00) 7 (9.72)
   B7 0 (0.00) 0 (0.00) 0 (0.00) 1 (7.14) 1 (1.38)
   B8 - - - - -
   B9 - - - - -
   B10 - - - - -
C C1—C4 C1 2 (9.52) 2 (10) 2 (11.76) 1 (7.14) 7 (9.72)
   C2 2 (9.52) 1 (5) 1 (5.88) 1 (7.14) 5 (6.94)
   C3 1(4.76) 1 (5) 0 (0.00) 1 (7.14) 3 (4.16)
   C4 2 (9.52) 1 (5) 1 (5.88) 1 (7.14) 5 (6.94)
   C5 0 (0.00) 0 (0.00) 1 (5.88) 1 (7.14) 2 (2.77)
   C6 - - - - -
M M1 M1 0 (0.00) 0 (0.00) 1 (5.88) 1 (7.14) 2 (2.77)
Total frequency (%)    21 (29.16) 20 (27.77) 17 (23.61) 14 (19.44) 72 (35.64)
Mean ± SE    1.11 ± 0.17 1.05 ± 0.19 0.89 ± 0.15 0.74 ± 0.13  
Kruskal wallis test    p < 0.05  
  1. n = The number of subtypes of alleles that bear Pst 1 RFLP pattern.