Figure 4

Comparison of samples with positive AMA-1 or MSP-1 responses. The scatterplot of AMA-1 and MSP-1 responses (average absorbance at 450 nm) is shown along with the reference lines denoting positive AMA-1 or MSP-1 responses (dotted lines) using the threshold absorbance values for previous infection of 0.319 and 0.260 absorbance units, respectively. Samples were positive for AMA-1 and MSP-1 (region I), MSP-1 positive and AMA-1 negative (region II), AMA-1 positive and MSP-1 negative (region III), or negative for both AMA-1 and MSP-1 (region IV). For clarity, samples with an absorbance value above 1.8 AU are shown as having a maximum value of 1.8 AU and those with no positive response to either antigen (region IV) are not shown.