Scatter plot analysis of kdr mutations in An. sinensis detected by AllGlo-qPCR. Endpoint fluorescence intensities between (A) FAM and VIC/HEX; (B) FAM and CY5; (C) VIC/HEX and CY5. PCR was performed in 96-well plates. The FAM, VIC/HEX and CY5 fluorescence intensities were measured and corrected for background and then plotted in a bi-directional scatter plot using Microsoft Excel. NTC: no template control.