|
Genotype
|
Detection assay*
|
Sensitivity (95% confidence level)
|
Specificity (95% confidence level)
|
|---|
| |
Sequencing
|
AS-PCR
|
TaqMan- qPCR
|
AllGlo-qPCR
|
AS-PCR
|
TaqMan- qPCR
|
AllGlo-qPCR
|
AS-PCR
|
TaqMan- qPCR
|
AllGlo-qPCR
|
|---|
|
TTG/TTG
|
62
|
59
|
62
|
62
|
95% (87%,98%)
|
100% (94%,100%)
|
100% (94%,100%)
|
100% (96%,100%)
|
100% (96%,100%)
|
100% (96%,100%)
|
|
TTT/TTT
|
61
|
60a
|
56
|
59
|
89% (78%,94%)
|
92% (82%,96%)
|
97% (89%,99%)
|
94% (87%,97%)
|
100% (96%,100%)
|
100% (96%,100%)
|
|
TGT/TGT
|
1
|
2b
|
1
|
1
|
100% (21%,100%)
|
100% (21%,100%)
|
100% (21%,100%)
|
99% (96%,99.9%)
|
100% (98%,100%)
|
100% (98%,100%)
|
|
TTG/TTT
|
3
|
5c
|
3
|
3
|
67% (21%,94%)
|
100% (44%,100%)
|
100% (44%,100%)
|
98% (94%,99%)
|
100% (98%,100%)
|
100% (98%,100%)
|
|
TTG/TGT
|
1
|
2d
|
1
|
1
|
100% (21%,100%)
|
100% (21%,100%)
|
100% (21%,100%)
|
99% (96%,99.9%)
|
100% (98%,100%)
|
100% (98%,100%)
|
|
TTT/TGT
|
30
|
30e
|
35f
|
32g
|
77% (59%,88%)
|
100% (89%,100%)
|
100% (89%,100%)
|
95% (89%,97%)
|
96% (91%,98%)
|
98% (94%,99.6%)
|
|
Total
|
158
|
158
|
158
|
158
| | | | | | |
|
Accuracy**
|
-
|
88.61%
|
96.84%
|
98.73%
| | | | | | |
- *:The superscript letter indicates an incorrect result generated by AS-PCR, TaqMan-qPCR or AllGlo-qPCR, compared to standard sequencing, as follows: a: 6 TTT/TGT; b: 1 TTT/TGT; c: 3 TTG/TTG; d: 1 TTG/TTT; e: 7 TTT/TTT; f: 5 TTT/TTT; g: 2 TTT/TTT.
- **:The accuracy refers to the percentage of correct results generated by AS-PCR, TaqMan-qPCR or AllGlo-qPCR out of 158 known kdr genotype samples.
