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Table 1 Comparison of kdr mutations in An. sinensis detected by AS-PCR, TaqMan -qPCR and AllGlo-qPCR versus direct sequencing

From: Development and application of an AllGlo probe-based qPCR assay for detecting knockdown resistance (kdr) mutations in Anopheles sinensis

Genotype

Detection assay*

Sensitivity (95% confidence level)

Specificity (95% confidence level)

 

Sequencing

AS-PCR

TaqMan- qPCR

AllGlo-qPCR

AS-PCR

TaqMan- qPCR

AllGlo-qPCR

AS-PCR

TaqMan- qPCR

AllGlo-qPCR

TTG/TTG

62

59

62

62

95% (87%,98%)

100% (94%,100%)

100% (94%,100%)

100% (96%,100%)

100% (96%,100%)

100% (96%,100%)

TTT/TTT

61

60a

56

59

89% (78%,94%)

92% (82%,96%)

97% (89%,99%)

94% (87%,97%)

100% (96%,100%)

100% (96%,100%)

TGT/TGT

1

2b

1

1

100% (21%,100%)

100% (21%,100%)

100% (21%,100%)

99% (96%,99.9%)

100% (98%,100%)

100% (98%,100%)

TTG/TTT

3

5c

3

3

67% (21%,94%)

100% (44%,100%)

100% (44%,100%)

98% (94%,99%)

100% (98%,100%)

100% (98%,100%)

TTG/TGT

1

2d

1

1

100% (21%,100%)

100% (21%,100%)

100% (21%,100%)

99% (96%,99.9%)

100% (98%,100%)

100% (98%,100%)

TTT/TGT

30

30e

35f

32g

77% (59%,88%)

100% (89%,100%)

100% (89%,100%)

95% (89%,97%)

96% (91%,98%)

98% (94%,99.6%)

Total

158

158

158

158

      

Accuracy**

-

88.61%

96.84%

98.73%

      
  1. *:The superscript letter indicates an incorrect result generated by AS-PCR, TaqMan-qPCR or AllGlo-qPCR, compared to standard sequencing, as follows: a: 6 TTT/TGT; b: 1 TTT/TGT; c: 3 TTG/TTG; d: 1 TTG/TTT; e: 7 TTT/TTT; f: 5 TTT/TTT; g: 2 TTT/TTT.
  2. **:The accuracy refers to the percentage of correct results generated by AS-PCR, TaqMan-qPCR or AllGlo-qPCR out of 158 known kdr genotype samples.