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Table 1 Comparison of kdr mutations in An. sinensis detected by AS-PCR, TaqMan -qPCR and AllGlo-qPCR versus direct sequencing

From: Development and application of an AllGlo probe-based qPCR assay for detecting knockdown resistance (kdr) mutations in Anopheles sinensis

Genotype Detection assay* Sensitivity (95% confidence level) Specificity (95% confidence level)
  Sequencing AS-PCR TaqMan- qPCR AllGlo-qPCR AS-PCR TaqMan- qPCR AllGlo-qPCR AS-PCR TaqMan- qPCR AllGlo-qPCR
TTG/TTG 62 59 62 62 95% (87%,98%) 100% (94%,100%) 100% (94%,100%) 100% (96%,100%) 100% (96%,100%) 100% (96%,100%)
TTT/TTT 61 60a 56 59 89% (78%,94%) 92% (82%,96%) 97% (89%,99%) 94% (87%,97%) 100% (96%,100%) 100% (96%,100%)
TGT/TGT 1 2b 1 1 100% (21%,100%) 100% (21%,100%) 100% (21%,100%) 99% (96%,99.9%) 100% (98%,100%) 100% (98%,100%)
TTG/TTT 3 5c 3 3 67% (21%,94%) 100% (44%,100%) 100% (44%,100%) 98% (94%,99%) 100% (98%,100%) 100% (98%,100%)
TTG/TGT 1 2d 1 1 100% (21%,100%) 100% (21%,100%) 100% (21%,100%) 99% (96%,99.9%) 100% (98%,100%) 100% (98%,100%)
TTT/TGT 30 30e 35f 32g 77% (59%,88%) 100% (89%,100%) 100% (89%,100%) 95% (89%,97%) 96% (91%,98%) 98% (94%,99.6%)
Total 158 158 158 158       
Accuracy** - 88.61% 96.84% 98.73%       
  1. *:The superscript letter indicates an incorrect result generated by AS-PCR, TaqMan-qPCR or AllGlo-qPCR, compared to standard sequencing, as follows: a: 6 TTT/TGT; b: 1 TTT/TGT; c: 3 TTG/TTG; d: 1 TTG/TTT; e: 7 TTT/TTT; f: 5 TTT/TTT; g: 2 TTT/TTT.
  2. **:The accuracy refers to the percentage of correct results generated by AS-PCR, TaqMan-qPCR or AllGlo-qPCR out of 158 known kdr genotype samples.