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Table 2 Comparison of four kdr genotyping assays based on the need for specialized equipment, cost, safety, simplicity and speed

From: Development and application of an AllGlo probe-based qPCR assay for detecting knockdown resistance (kdr) mutations in Anopheles sinensis

Method

Equipment required

Hazardous chemicals

Protocol run time

No. of steps

Primers/probe required

No. of tubes required per sample

Cost per run*

Sequencing

PCR thermocycler

Ethidium bromide

6 h

2

2 PCR primers

1

$4.20

 

Gel electrophoresis and imaging equipment

      
 

Nucleic acid sequencing machine

      

AS-PCR

PCR thermocycler

Ethidium bromide

4 h 30 min

2

5 PCR primers

2

$0.25

 

Gel electrophoresis and imaging equipment

      

TaqMan-qPCR

Real-time PCR machine

-

1 h 45 min

1

2 PCR primers

2

$0.70

     

3 fluorescently labelled probes

  

AllGlo-qPCR

Real-time PCR machine

-

1 h 45 min

1

2 PCR primers

1

$0.45

     

3 fluorescently labelled probes

  
  1. *:Refers to the average cost to run one sample; the cost of the equipment was not included.