Method | Equipment required | Hazardous chemicals | Protocol run time | No. of steps | Primers/probe required | No. of tubes required per sample | Cost per run* |
---|---|---|---|---|---|---|---|
Sequencing | PCR thermocycler | Ethidium bromide | 6Â h | 2 | 2 PCR primers | 1 | $4.20 |
 | Gel electrophoresis and imaging equipment |  |  |  |  |  |  |
 | Nucleic acid sequencing machine |  |  |  |  |  |  |
AS-PCR | PCR thermocycler | Ethidium bromide | 4Â h 30Â min | 2 | 5 PCR primers | 2 | $0.25 |
 | Gel electrophoresis and imaging equipment |  |  |  |  |  |  |
TaqMan-qPCR | Real-time PCR machine | - | 1Â h 45Â min | 1 | 2 PCR primers | 2 | $0.70 |
 |  |  |  |  | 3 fluorescently labelled probes |  |  |
AllGlo-qPCR | Real-time PCR machine | - | 1Â h 45Â min | 1 | 2 PCR primers | 1 | $0.45 |
 |  |  |  |  | 3 fluorescently labelled probes |  |  |