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Table 2 Comparison of four kdr genotyping assays based on the need for specialized equipment, cost, safety, simplicity and speed

From: Development and application of an AllGlo probe-based qPCR assay for detecting knockdown resistance (kdr) mutations in Anopheles sinensis

Method Equipment required Hazardous chemicals Protocol run time No. of steps Primers/probe required No. of tubes required per sample Cost per run*
Sequencing PCR thermocycler Ethidium bromide 6 h 2 2 PCR primers 1 $4.20
  Gel electrophoresis and imaging equipment       
  Nucleic acid sequencing machine       
AS-PCR PCR thermocycler Ethidium bromide 4 h 30 min 2 5 PCR primers 2 $0.25
  Gel electrophoresis and imaging equipment       
TaqMan-qPCR Real-time PCR machine - 1 h 45 min 1 2 PCR primers 2 $0.70
      3 fluorescently labelled probes   
AllGlo-qPCR Real-time PCR machine - 1 h 45 min 1 2 PCR primers 1 $0.45
      3 fluorescently labelled probes   
  1. *:Refers to the average cost to run one sample; the cost of the equipment was not included.