Figure 3From: Humanized HLA-DR4.RagKO.IL2RγcKO.NOD (DRAG) mice sustain the complex vertebrate life cycle of Plasmodium falciparum malariaDRAG mice infused with human HSC develop human RBCs. A) Enumeration of human erythrocytes (hEry) and reticulocytes (hRet) in peripheral blood of DRAG mice by FACS using anti-human CD235a (glycophorin A) and Retic-Count (left panel). Controls are DRAG mice non-infused with HSCs (upper right panel) and human blood (lower right panel). B) Immunostaining of peripheral blood from HSC-infused DRAG mice (right) and human blood (left) with anti-human haemoglobin A. Arrow shows the presence of human RBCs. C) Frequency of human erythrocytes and reticulocytes in blood of HSC-infused DRAG mice (n = 10). Data represent mean ± SD of mice analysed individually. D) Bone marrow from HSC-infused (n = 3) and control (non-infused) (n = 7) DRAG mice was stained with anti-human CD235a and anti-mouse Ter-119 (upper left panels). Data represent mean ± SD of mice analysed individually. Staining with anti-human CD235a and anti-human CD71 showed that that the nucleated human RBC precursors expressed CD71 (upper right panel) (n = 1). Annexin-V and 7-AAD staining of human RBC precursors (CD235+) and non-erythroctyc precursors (CD235−) in the bone marrow of HSC-infused DRAG mice (lower panels) (n = 1).Back to article page