Figure 2

CPO gives a better distinction between early rings and uninfected erythrocytes compared to mitotracker red. Plasmodium falciparum NF54 asynchronous culture at ~4.0% parasitaemia (microscopy estimate), 2.5% haematocrit was co-stained with 20 nM CPO and 5 μM. Dot plots of SS Log/FL1-CPO for uRBCs (A1) and iRBCs (A2), respectively, are shown. There is a clear distinction between iRBCs carrying early ring parasite stages and uRBCs. Also, dot plots of SS Log/FL3-mitotracker red for uRBCs (B1) and iRBCs (B2), respectively, are shown. There is a poor distinction between iRBCs carrying early rings parasite stages and uRBCs. Quadrants are labeled ‘G’ for CPO and ‘K’ for mitotracker red plots respectively. The positive (+) and negative (-) signs denote the presence or absence respectively of a given dye signal or cell attribute in the quadrant. (C) Plot of FL1-CPO/FL3-mitotracker red. Infected RBCs containing live parasites are identified as mitotracker red-CPO double positive cells (3.49%).