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Figure 3 | Malaria Journal

Figure 3

From: High-throughput tri-colour flow cytometry technique to assess Plasmodium falciparum parasitaemia in bioassays

Figure 3

Dynamics of mitotracker red and CPO staining of compromised parasites. Plasmodium falciparum NF54 asynchronous culture at 2% parasitaemia was treated with 20 nM atovaquone/1 mM proguanil to induce compromised state parasites. A) Flow cytometry parasitaemia estimate by staining individually with mitotracker red (5 μM) and CPO (20 nM) at 0 hrs, 24 hrs and 48 hrs post treatment. Values are plotted ± standard deviation for triplicate wells. B) Representative Z-stack images (interval = 0.2 μm) by mitotracker red-CPO staining of culture corresponding to 0 hrs, 24 hrs and 48 hrs post treatment. Each row represents a sampling time and shows, from left to right mitotracker red staining, CPO staining and the merged image.

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