Table 1 Efficacy of intravenous methylene blue, intravenous artesunate, and their combination in the Plasmodium berghei -infected rat

Dose (mg/kg/day × 3 days)

0

5

10

20

40

80

Number of rats

6

7

7

7

8

7

Number of rats cleared*

0/6

0/7

0/7

7/7

8/8

PCT (mean hour)

52

48

Mortality (dead/total)

1/6

1/7

0/7

0/7

0/8

7/7

B) Artesunate alone

Dose (mg/kg/day × 3 days)

0

15

30

60

120

240

480

Number of rats

6

7

6

6

6

6

5

Number of rats cleared*

0/6

0/7

0/6

6/6

6/6

6/6

PCT (mean hour)

59

48

49

Mortality (dead/total)

1/6

1/7

0/6

0/6

0/6

0/6

4/5

C) Methylene blue in combination with artesunate

Dose (mg/kg/day × 3 days)

MB5/AS 15

MB10/AS30

MB20/AS60

MB40/AS120

MB40/AS240

Number of rats

8

8

12

6

5

Number of rats cleared*

4/8

8/8

12/12

6/6

PCT (mean hour)

61

46

45

36

Mortality (dead/total)

0/8

0/8

0/12

0/6

4/5