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Table 2 Absolute recovery, using response factor

From: The development and validation of an LC-MS/MS method for the determination of a new anti-malarial compound (TK900D) in human whole blood and its application to pharmacokinetic studies in mice

Sample Analyte conc. (ng/ml) Mean of peak areas Absolute recovery (%) CV (%)
After extraction Theoretical values
High conc. 800.0 825850 1120664 73.7 4.3
Medium conc. 160.1 169317 260280 65.1 4.5
Low conc. 10.01 10482 14370 72.9 8.9
Mean 70.6 5.9
ISTD 100.0 418683 543089 77.1 2.8
  1. N.B.: The concentration of the ISTD was same at high, medium and low concentration levels.