The development and validation of an LC-MS/MS method for the determination of a new anti-malarial compound (TK900D) in human whole blood and its application to pharmacokinetic studies in mice

Table 2 Absolute recovery, using response factor

Sample	Analyte conc. (ng/ml)	Mean of peak areas		Absolute recovery (%)	CV (%)
Sample	Analyte conc. (ng/ml)	After extraction	Theoretical values	Absolute recovery (%)	CV (%)
High conc.	800.0	825850	1120664	73.7	4.3
Medium conc.	160.1	169317	260280	65.1	4.5
Low conc.	10.01	10482	14370	72.9	8.9
Mean				70.6	5.9
ISTD	100.0	418683	543089	77.1	2.8

N.B.: The concentration of the ISTD was same at high, medium and low concentration levels.

ISSN: 1475-2875