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Table 3 Stability assessment

From: The development and validation of an LC-MS/MS method for the determination of a new anti-malarial compound (TK900D) in human whole blood and its application to pharmacokinetic studies in mice

Stability Analyte code Mean analyte peak area (n = 6)
Room temperature ~ 5°C ~ -20°C Fresh (reference)
Analyte stock solution stability in methanol TK900D Peak area 813083 800550 762900 760700
% Reference 106.9 105.2 100.3 N/A
% CV 2.9 1.4 2.4 1.8
TK900E Peak area 876300 881567 836667 852133
% Reference 102.8 103.5 98.2 N/A
   % CV 1.9 2.8 2.2 2.9
Stability TK900D Nominal concentration (ng/ml)
High (800.0) Low (10.01)
Long term Mean 805.7 9.598
% CV 6.9 11.9
% Bias 0.7 -4.0
Freeze and thaw Mean 852.7 10.87
% CV 5.8 8.9
% Bias 6.6 8.6
On bench Mean 866.0 10.53
% CV 3.4 7.5
% Bias 8.3 5.2
OIS Mean 806.9 10.46
% CV 0.6 1.4
% Bias 0.9 4.5
  1. All results are mean of n = 6.