Table 2 PCR conditions employed for various SNPs
SNP | PCR conditions | |||||
|---|---|---|---|---|---|---|
Initial denaturation | Denaturation | Annealing | Extension | Final extension | Hold temp | |
CYP3A4*1B | 94°C for 2 min | 94°C for 30 s (30 cycles) | 60°C for 30 s (30 cycles) | 72°C for 90 s (30 cycles) | 72°C for 5 min | 4°C |
CYP2B6*6 | 96°C for 3 min | 96°C for 30 s (40 cycles) | 64°C for 1 min 30 s (40 cycles) | 72°C for 1 min 30 s (40 cycles) | 72°C for 10 min | 4°C |
CYP3A5*3 | 96°C for 3 min | 96°C for 30 s (40 cycles) | 59.3°C for 30 s (40 cycles) | 72°C for 30 s (40 cycles) | 72°C for 10 min | 4°C |
CYP2C8*2 | 96°C for 3 min | 96°C for 30 s (40 cycles) | 56°C for 1 min (40 cycles) | 72°C for 1 min (40 cycles) | 72°C for 10 min | 4°C |
CYP2C8*3 | 96°C for 3 min | 96°C for 30 s (30 cycles) | 64°C 1 min 30 s (30 cycles) | 72°C 1 min 30 s (30 cycles) | 72°C for 10 min | 4°C |