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Figure 1 | Malaria Journal

Figure 1

From: Characterization of the Anopheles gambiae octopamine receptor and discovery of potential agonists and antagonists using a combined computational-experimental approach

Figure 1

Phylogenetic relationship of Anopheles gambiae AgOAR45A and AgOAR45B with other octopamine (OAR) and tyramine (TyrR) insect receptors. Sequences from An. gambiae (AGAP000045, AGAP002519, AGAP002888, AGAP002886, AGAP004034, AGAP013324; Ae. aegypti (AAEL004398, AAEL004396, AAEL006844, AAEL014224, AAEL016990, AAEL005952, AAEL005945), and Cu. quinquefasciatus (CPIJ019015) were downloaded from VectorBase. Sequences from D. melanogaster [DmTyrRII (CG16766), DmTyrR (CG7431), DmOct-TyrR (CG7485), DmOamb-B (CG3856-RB), DmOamb-D (CG3856-RD), DmOAbeta1 (CG6919), DmOAbeta3 (CG42244) and DmOAbeta2 (CG33976)] were downloaded from FlyBase. Additional sequences, from Bombyx mori [BmOA1 (NP_001091748.1)], Apis mellifera [AmOA1 (NP_001011565.1)], and Tribolium [TcOAR1 (DAA64496.1)] were downloaded from GenBank. The alignment was performed on complete amino acid sequences and calculated using the ClustalX 2.1 [49]. The tree was constructed using ClustalX 2.1, and the neighbor-joining algorithm with a bootstrap value of 1,000. Numbers on branches are the percentage of bootstrap support for each branch node, only those above 50% are represented on the trees. The scale represents the rate of amino acid substitution per site. AgOAR45A and Ag OAR45B are in bold.

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Malaria Journal

ISSN: 1475-2875

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