Changes in metabolic phenotypes of Plasmodium falciparum in vitro cultures during gametocyte development

Malaria Journal

Table 1 Metabolites significantly altered by Plasmodium falciparum infection throughout gametocyte culture period

	Metabolite	p-values
		Day 1	Day 4	Day 7	Day 8	Day 14
Metabolites released into medium	Acetate	0.0046	0.0005	0.0008	0.0024	0.0024
	Alanine	0.0012	0.0005	0.0008	NS	0.0188 (-)
	Glycerol	0.0012	0.0005	0.0153	NS	NS
	Histidine	0.0129	0.0005	0.0008	0.0036	NS
	Lactate	0.0012	0.0005	0.0008	0.0024	0.0024 (-)
	Ornithine	0.0129	NS	NS	NS	NS
	Phenylalanine	0.0014	0.0005	0.0008	0.0188	NS
	Pyroglutamate	NS	NS	NS	0.0444 (-)	0.0210 (-)
	Pyruvate	0.0012	0.0005	0.0008	0.0024	0.0250 (-)
	Tyrosine	0.0046	0.0007	0.0090	NS	NS
	Valine	0.0033	0.0005	0.0153	NS	NS
Metabolites consumed from medium	Arginine	0.0166 (-)	NS	NS	NS	NS
	Glucose	NS	0.0005	0.0038	0.0091	0.0152
	Glutamine	0.0033 (-)	NS	NS	NS	NS
	Hypoxanthine	NS	NS	0.0125 (-)	NS	NS
	myo-Inositol	NS	NS	NS	NS	0.0210 (-)
	Isoleucine	NS	0.0012	NS	NS	0.0250
	Lipid/Sugar CH = CH	NS	NS	NS	NS	NS
	Lipid CH = CH-CH2-CH2	NS	NS	NS	NS	0.0414
	Lipid CH2	NS	NS	NS	NS	0.0414
	Lipid CH2-CH2-CO	0.0222 (-)	NS	NS	NS	NS
	Lipid CH2-CO	0.0033 (-)	NS	NS	NS	NS
	Lipid CH3	0.0397 (-)	NS	NS	NS	0.0180
Variable consumption/release	Leucine	0.0065 (-)	0.0005	0.0296	NS	0.0188 (-)

Statistical differences between metabolite levels of medium from parasite cultures and uninfected erythrocytes, assessed for each time-point as measured via Mann-Whitney U-test. P-values indicate significantly higher metabolite release/consumption by parasite cultures, unless marked with (-), where release/consumption was lower than erythrocytes. NS, no significant difference.

ISSN: 1475-2875