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Figure 6 | Malaria Journal

Figure 6

From: The dimerization domain of Pf CENP-C is required for its functions as a centromere protein in human malaria parasite Plasmodium falciparum

Figure 6

In vivo enrichment of Pf CENP-C on the Plasmodium centromeres and its in vivo interaction with Pf CENH3 and mitotic spindles. (A) ChIP-PCR assay showing the enrichment of Pf CENP-C on the six P. falciparum centromeres (CEN 1, 2, 3, 4, 9, and 12) as compared to the non centromeric regions (red dotted boxes). +CEN and –CEN represents the non-centromeric regions lying upstream and downstream of each P. falciparum centromere, respectively. The non-centromeric regions show weak or no recruitment of Pf CENH3 and Pf CENP-C for all the six P. falciparum centromeres tested. The fold enrichment (ChIP over input) was determined for each centromere in comparison with the non-centromeric regions by quantifying the gel band intensities using AlphaImager. In the graphs (x-axis), CENH3 (+1), CENP-C (+1) and CENH3 (-1), CENP-C (-1) represents the ChIP with Pf CENH3 and Pf CENP-C at regions upstream and downstream to the centromeres, respectively. CENH3 (0) and CENP-C (0) in the x-axis of the graphs represent the Pf CENH3 and Pf CENP-C enriched centromeric regions, respectively. The y-axis represents the fold enrichment (ChIP over input) for each chromosome tested. (B) Immunoprecipitation assays with Pf CENP-C (panels, i-iii) and Pf CENH3 (panels, iv-vi) show the interaction of Pf CENP-C with Pf CENH3 (panel ii) and mitotic spindles (panel iii). Pf CENH3 also interacts with Pf CENP-C (panel v) and tubulin (panel vi).

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