Dose–response relationship for primaquine in reducing the duration of (female) gametocyte carriage assessed by Pfs25 transcripts. Mean (95%CI) duration of female gametocyte carriage in Ugandan children with falciparum malaria treated with artemether-lumefantrine (AL) and different doses of primaquine as reported by Eziefula et al.. Duration was estimated by fitting of a deterministic compartmental mathematical model to repeated Pfs25 quantitative real time nucleic acid sequence- based analysis gametocyte prevalence estimates. The vertical dashed line indicates the set threshold for non- inferiority compared with the primaquine 0.75 mg/kg reference group (non-inferiority margin of 2.5 days). The currently recommended 0.25 mg/kg dose is indicated by the horizontal line.