Generation and purity of recombinant PfRON3 protein. (A) SDS-PAGE analysis of purified His- PfRON3-III protein, which was generated using an E. coli expression system. The purified His-PfRON3-III (66.1 kDa) protein was resolved on a 12% SDS-PAGE gel and stained with Coomassie brilliant blue R-250. (B) Western blotting analysis of purified His-PfRON3-III protein under reducing conditions with anti-His-tag IgG. The smaller bands are degraded products of the full-length protein.