Cumulative survival analysis (Kaplan-Meier method). On day 5 pi, 6 pi or 7 pi, mice received a single IN administration of artesunate (20 mg/kg). A control group received IN placebo on day 5 pi, 6 pi or 7 pi. The endpoint was day 12 pi. The log-rank test revealed a significant reduction of the mortality in all treated groups compared with control group (day 5 pi: p = 1.58 10−7, day 6 pi: p = 8.89 10−4, day 7 pi: p = 4.92 10−5). The efficiency of the treatment was similar in the three treated groups (p > 0.05). ***: p <0.001, NS: non-significant, coloured arrows indicates the time of treatment for each group, black line: control group, blue line: mice treated at day 5, red line: mice treated at day 6, green line: mice treated at day 7.