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Table 1 MSP2 D and C domain epitope mapping in different endemic areas

From: Plasmodium falciparum merozoite surface protein 2: epitope mapping and fine specificity of human antibody response against non-polymorphic domains

  Peptides PNG (N = 42) Tanzania (N = 37) BF (N = 49) Mali (N = 39) Sum of positves: n (%) p value
Positive: n (%) OD mean SD Positive: n (%) OD mean SD Positive: n (%) OD mean SD Positive: n (%) OD mean SD
3D7 family D 41 (98) 2.24 1.29 33 (89) 1.47 1.29 49 (100) 2.01 0.74 38 (97) 1.75 1.32 161 (96) 0.058
P11 2 (5) 0.28 0.11 5 (14) 0.22 0.09 13 (27) 0.42 0.69 4 (10) 0.14 0.07 24 (14) 0.011
P12 13 (31) 0.55 0.61 8 (22) 0.30 0.25 16 (33) 0.49 0.53 16 (41) 0.49 0.91 53 (32) 0.120
P13 28 (67) 0.85 1.03 19 (51) 0.53 0.95 19 (39) 0.61 0.78 18 (46) 0.54 0.91 84 (50) 0.125
P14 19 (45) 0.64 0.68 10 (27) 0.50 0.83 28 (57) 1.09 1.25 10 (25) 0.29 0.43 67 (40) 0.003
P15 17 (40) 0.60 0.67 9 (24) 0.40 0.60 22 (45) 0.82 0.95 10 (25) 0.40 0.60 58 (35) 0.107
P16 14 (33) 0.60 0.64 9 (24) 0.57 1.19 27 (55) 0.85 0.84 18 (46) 0.60 0.89 68 (41) 0.028
P17 8 (19) 0.30 0.27 10 (27) 0.47 0.92 34 (69) 0.96 0.93 6 (15) 0.24 0.24 58 (35) <0.001
P18 1 (2) 0.24 0.07 13 (35) 0.28 0.23 14 (29) 0.48 0.68 5 (13) 0.23 0.50 33 (20) 0.001
P19 3 (7) 0.22 0.10 8 (22) 0.25 0.23 3 (6) 0.23 0.17 4 (10) 0.18 0.24 18 (11) 0.115
FC27 family D 40 (90) 1.21 0.82 31 (84) 1.01 0.81 42 (86) 1.47 1.11 25 (65) 0.72 1.06 138 (83) 0.009
P23 20 (48) 0.40 0.49 7 (19) 0.23 0.11 16 (33) 0.64 0.88 5 ()13 0.28 0.69 48 (29) 0.002
P24 3 (8) 0.24 0.22 3 (8) 0.19 0.17 11 (22) 0.32 0.21 4 (10) 0.27 0.49 21 (12) 0.114
P25 15 (36) 0.39 0.58 9 (24) 0.44 0.50 22 (45) 0.54 0.63 7 (18) 0.17 0.12 53 (32) 0.035
P26 17 (40) 0.32 0.29 11 (30) 0.36 0.58 11 (22) 0.33 0.31 5 (13) 0.16 0.11 44 (26) 0.018
P27 3 (8) 0.17 0.06 4 (11) 0.27 0.44 4 (8) 0.25 0.11 2 (5) 0.17 0.26 13 (8) 0.806
C region C 29 (69) 0.57 0.31 26 (70) 0.58 0.62 33 (67) 0.76 0.67 16 (41) 0.34 0.31 104 (62) 0.061
P28 2 (5) 0.20 0.10 0 (0) 0.22 0.05 4 (8) 0.23 0.13 1 (2) 0.16 0.17 7 (4) 0.384
P29 21 (50) 0.40 0.31 7 (19) 0.41 0.56 19 (39) 0.41 0.32 5 (13) 0.15 0.08 52 (31) 0.001
P30 16 (38) 0.23 0.11 16 (43) 0.40 0.64 13 (27) 0.39 0.39 5 (13) 0.19 0.17 50 (30) 0.017
  1. Table 1 Showed ELISA performed on LSPs and 20 mers covering the D and C fragments of the two allelic families of MSP2. Adult plasma from PNG (N = 42), TZ (N = 37), BF (N = 49) and Mali (N = 39) were obtained during malaria season transmission. ELISA was considered positive if the ratio of mean OD of test sample/mean OD of negative control was more than or equal to 2. Peptides in bold indicate the major recognized 20 mers. Means of Ab OD, standard deviation (SD), and p-values from Chi-square tests (comparing the proportion of responders among the four endemic areas for each peptide) were determined. P values ≤ 0.05 are in bold and considered statically significant.