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Table 1 MSP2 D and C domain epitope mapping in different endemic areas

From: Plasmodium falciparum merozoite surface protein 2: epitope mapping and fine specificity of human antibody response against non-polymorphic domains

 

Peptides

PNG (N = 42)

Tanzania (N = 37)

BF (N = 49)

Mali (N = 39)

Sum of positves: n (%)

p value

Positive: n (%)

OD mean

SD

Positive: n (%)

OD mean

SD

Positive: n (%)

OD mean

SD

Positive: n (%)

OD mean

SD

3D7 family

D

41 (98)

2.24

1.29

33 (89)

1.47

1.29

49 (100)

2.01

0.74

38 (97)

1.75

1.32

161 (96)

0.058

P11

2 (5)

0.28

0.11

5 (14)

0.22

0.09

13 (27)

0.42

0.69

4 (10)

0.14

0.07

24 (14)

0.011

P12

13 (31)

0.55

0.61

8 (22)

0.30

0.25

16 (33)

0.49

0.53

16 (41)

0.49

0.91

53 (32)

0.120

P13

28 (67)

0.85

1.03

19 (51)

0.53

0.95

19 (39)

0.61

0.78

18 (46)

0.54

0.91

84 (50)

0.125

P14

19 (45)

0.64

0.68

10 (27)

0.50

0.83

28 (57)

1.09

1.25

10 (25)

0.29

0.43

67 (40)

0.003

P15

17 (40)

0.60

0.67

9 (24)

0.40

0.60

22 (45)

0.82

0.95

10 (25)

0.40

0.60

58 (35)

0.107

P16

14 (33)

0.60

0.64

9 (24)

0.57

1.19

27 (55)

0.85

0.84

18 (46)

0.60

0.89

68 (41)

0.028

P17

8 (19)

0.30

0.27

10 (27)

0.47

0.92

34 (69)

0.96

0.93

6 (15)

0.24

0.24

58 (35)

<0.001

P18

1 (2)

0.24

0.07

13 (35)

0.28

0.23

14 (29)

0.48

0.68

5 (13)

0.23

0.50

33 (20)

0.001

P19

3 (7)

0.22

0.10

8 (22)

0.25

0.23

3 (6)

0.23

0.17

4 (10)

0.18

0.24

18 (11)

0.115

FC27 family

D

40 (90)

1.21

0.82

31 (84)

1.01

0.81

42 (86)

1.47

1.11

25 (65)

0.72

1.06

138 (83)

0.009

P23

20 (48)

0.40

0.49

7 (19)

0.23

0.11

16 (33)

0.64

0.88

5 ()13

0.28

0.69

48 (29)

0.002

P24

3 (8)

0.24

0.22

3 (8)

0.19

0.17

11 (22)

0.32

0.21

4 (10)

0.27

0.49

21 (12)

0.114

P25

15 (36)

0.39

0.58

9 (24)

0.44

0.50

22 (45)

0.54

0.63

7 (18)

0.17

0.12

53 (32)

0.035

P26

17 (40)

0.32

0.29

11 (30)

0.36

0.58

11 (22)

0.33

0.31

5 (13)

0.16

0.11

44 (26)

0.018

P27

3 (8)

0.17

0.06

4 (11)

0.27

0.44

4 (8)

0.25

0.11

2 (5)

0.17

0.26

13 (8)

0.806

C region

C

29 (69)

0.57

0.31

26 (70)

0.58

0.62

33 (67)

0.76

0.67

16 (41)

0.34

0.31

104 (62)

0.061

P28

2 (5)

0.20

0.10

0 (0)

0.22

0.05

4 (8)

0.23

0.13

1 (2)

0.16

0.17

7 (4)

0.384

P29

21 (50)

0.40

0.31

7 (19)

0.41

0.56

19 (39)

0.41

0.32

5 (13)

0.15

0.08

52 (31)

0.001

P30

16 (38)

0.23

0.11

16 (43)

0.40

0.64

13 (27)

0.39

0.39

5 (13)

0.19

0.17

50 (30)

0.017

  1. Table 1 Showed ELISA performed on LSPs and 20 mers covering the D and C fragments of the two allelic families of MSP2. Adult plasma from PNG (N = 42), TZ (N = 37), BF (N = 49) and Mali (N = 39) were obtained during malaria season transmission. ELISA was considered positive if the ratio of mean OD of test sample/mean OD of negative control was more than or equal to 2. Peptides in bold indicate the major recognized 20 mers. Means of Ab OD, standard deviation (SD), and p-values from Chi-square tests (comparing the proportion of responders among the four endemic areas for each peptide) were determined. P values ≤ 0.05 are in bold and considered statically significant.
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Malaria Journal

ISSN: 1475-2875

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