Skip to main content

Table 2 MSP2 D and C domains epitope mapping in different age groups

From: Plasmodium falciparum merozoite surface protein 2: epitope mapping and fine specificity of human antibody response against non-polymorphic domains

  Peptides Children (1) (≤5 years) N = 38 Adolescents (2) (6–14 years) N = 43 Adults (3) (≥15 years) N = 26 p value
Positive: n (%) Mean OD SD Positive: n (%) Mean OD SD Positive: n (%) Mean OD SD    
            1 v 2 1 v 3 2 v 3
3D7 family D 32 (84) 1.26 0.97 43 (100) 1.50 1.35 26 (100) 2.05 0.94 0.008 0.008 1
P11 0 (0) 0.11 0.01 2 (5) 0.12 0.02 8 (31) 0.17 0.11 0.148 0.001 0.007
P12 8 (21) 0.20 0.09 18 (42) 0.66 0.94 8 (31) 0.41 0.37 0.038 0.386 0.346
P13 16 (42) 0.64 0.95 20 (47) 0.44 0.73 11 (42) 0.40 0.37 0.690 0.987 0.733
P14 11 (29) 0.16 0.07 12 (28) 0.18 0.13 13 (50) 0.51 0.66 0.918 0.086 0.065
P15 13 (34) 0.18 0.13 24 (56) 0.39 0.53 9 (35) 0.59 0.83 0.045 0.973 0.078
P16 13 (34) 0.43 0.54 19 (44) 0.67 0.93 12 (46) 0.53 0.70 0.356 0.337 0.874
P17 5 (13) 0.20 0.16 22 (51) 0.21 0.16 7 (27) 0.29 0.35 <0.0001 0.181 0.036
P18 2 (5) 0.14 0.08 2 (5) 0.13 0.02 6 (23) 0.33 0.56 0.899 0.048 0.038
P19 14 (37) 0.22 0.31 4 (9) 0.11 0.01 2 (8) 0.20 0.27 0.002 0.002 0.814
FC27 family D 23 (61) 0.21 0.11 37 (86) 0.85 1.15 23 (88) 0.76 0.50 0.007 0.006 0.768
P23 2 (5) 0.11 0.02 12 (28) 0.50 0.94 9 (35) 0.15 0.06 0.003 0.003 0.562
P24 0 (0) 0.14 0.04 4 (9) 0.45 0.83 7 (27) 0.21 0.13 0.036 0.002 0.071
P25 4 (11) 0.13 0.05 7 (16) 0.20 0.17 10 (38) 0.19 0.12 0.444 0.009 0.045
P26 4 (11) 0.11 0.02 10 (23) 0.18 0.17 8 (31) 0.17 0.08 0.118 0.050 0.499
P27 6 (16) 0.21 0.36 6 (14) 0.11 0.02 2 (8) 0.20 0.29 0.817 0.305 0.400
C region C 14 (37) 0.27 0.28 19 (44) 0.25 0.12 14 (54) 0.50 0.40 0.500 0.175 0.435
P28 6 (16) 0.17 0.21 2 (5) 0.13 0.02 3 (23) 0.19 0.22 0.098 0.473 0.038
P29 5 (13) 0.12 0.04 11 (26) 0.13 0.04 8 (31) 0.18 0.11 0.150 0.096 0.644
P30 2 (5) 0.13 0.05 5 (12) 0.13 0.03 7 (27) 0.28 0.26 0.296 0.022 0.125
  1. Table 2 showed ELISA performed on LSPs and 20 mers covering the D and C fragments of the two allelic families of MSP2 using sera from age groups living in Mali. Plasma was used at dilution 1/200. The ELISA was considered positive if the ratio of mean Ab OD of test sample/mean OD of negative control was more than or equal to 2. Ab OD, SD and p-values from Fisher’s exact test (comparison of the proportions positive between two age groups) were calculated for each peptide. Numbers (1, 2, 3) represent respectively children, adolescents and adults code. v: versus. P values ≤ 0.05 are in bold and considered statically significant.