Immuno co-localization of BiP with MSP1 and DBP in schizont stages of Plasmodium vivax . (A) MSP1 is localized in close proximity to the plasma membrane of early schizonts and surrounding merozoite membranes in late-segmented schizonts. (B) BiP is distributed throughout the cytoplasm of early schizonts and concentrated at the apical end of the merozoite in late segmenting schizonts. (C) In late segmenting schizonts, BiP is localized at the apical end in close proximity to the nucleus of daughter merozoites while DBP is localized at the apical end next to BiP. (D) Pvs16 was detected only in morphologic forms identified as gametocytes, localizing mainly in the peripheral cytoplasm.