Gating strategy for BiP + events. Uninfected erythrocytes were used as a control for gating purpose. The events were gated based on the forward scatter and side scatter (FSC-A/SSC-A) profile in the dot plot (P1). The events in gate P1 were viewed in a FSC-A/FSC-H profile to distinguish and select cell singlet in gate P2. The single cells from gate P2 were viewed in a BiP Alexa 660-A/ FL2-A dot plot. The events in gate P3 were selected and viewed in BiP Alexa 660-A/ FL2-A dot plot. The BiP + events were obtained by subtracting the events in gate R1 with the events in gate P5.