Figure 1

The majority of recombinant merozoite extracellular proteins are expressed at their expected size. One microgram of each purified biotinylated merozoite protein (as estimated by the absorbance at 280 nm) was resolved under reducing conditions by SDS-PAGE, blotted, and probed using streptavidin-HRP. The expected molecular mass of each recombinant protein is indicated in brackets above each lane, including the Cd4-6xHis tag (25 kDa).