Silver-stained 2-D gels of Anopheles gambiae midgut proteins: (A) Sugar-fed females. (B) Blood-fed females. (C) Males. Black arrowheads: sugar-meal-dependent proteins (panel A), blood-meal-dependent proteins (panel B), male-specific proteins (panel C); white and black arrowheads: constitutive female-specific proteins (panels A and B); thin arrows: proteins detected in both sugar-fed females and males (panels A and C). Numbers refer to proteins described in Figure 2; lettered spots are discussed in the main text. The molecular weights of standards (kDa) are indicated on the right-hand side of the gels.