Uptake of high molecular weight proteins by infected human erythrocytes (A) Western blot analysis of biotin labelled proteins in different fractions of saponin lysed parasitized RBC and uninfected RBCs using streptavidin horseradish peroxidate conjugate. Protein markers (lane 1), saponin treatment lysate of uninfected RBCs incubated with labelled PfHRP-2 (lane 2), intact parasite pellet of infected RBCs incubated with unlabelled recombinant PfHRP-2 (lane 3), supernatant of infected RBCs treated with labelled PfHRP-2 (lane 4), parasite pellet of infected RBC incubated with labelled β galactosidase (lane 5), egg albumin (lane 6), β amylase (tetramer) (lane 7) and recombinant PfHRP-2 (lane 8). (B) SDS-PAGE analysis of radio-iodinated proteins imported by the parasite from the culture. Saponin lysed intact parasite pellet incubated with recombinant 125I PfHRP-2 (lane 1) and 125I egg albumin (lane 4). Supernatant of saponin lysed infected RBC incubated with 125I PfHRP-2 (lane 2) and 125I egg albumin (lane 3). (C) Direct immunofluorescence analysis to show the uptake of HAS by infected human erythrocytes. (A) uninfected RBCs incubated with biotin labelled protein and (B) infected RBCs incubated with biotin labelled protein.