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Figure 1 | Malaria Journal

Figure 1

From: Pilot survey of expressed sequence tags (ESTs) from the asexual blood stages of Plasmodium vivax in human patients

Figure 1

Overview of the pipeline used in the ESTs identification process. Sequences were automatically assessed for quality and removal of contaminants. BLAST similarity searches against PlasmoDB (PDB), GenBank (GB), and TIGR (preliminary sequence data was obtained from The Institute for Genomic Research through the website at http://www.tigr.org) data bases were performed after assembling the sequences with CAP3 [10] and masking regions of low complexity with the SEG [11] or DUST (Tatusov & Lipman, unpublished; http://blast.wustl.edu/pub/dust) programs. An E-value of < 10-30 for both BLASTN and BLASTX defined a significant database match.

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