Figure 2

Interaction of PfHsp90 with ATP and GA. PfHsp90 was allowed to bind to ATP-Sepharose [19] as described under Materials and Methods, with the following additions to the incubation mixture (Panel A): None (lane 1), 10 μg/ml GA (lane 2), 10 mM ATP (lane 3), 20 mM EDTA (lane 4), 10 mM GTP (lane 5). Lane 0 shows the input amount. The bound Hsp90 was analyzed by SDS-PAGE and Western as described under Materials and Methods. Panel A shows a representative immunoblot. Similar experiments were also performed with various concentrations of ADP, ATP, and GTP, and the amounts of Hsp90 bound to ATP-Sepharose were determined from densitometric scan of the blot and plotted in Panel B.