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Table 8 Prediction of DAQ IC50 nM value for CQ-resistant lines on the basis of mean EIS for the 72-76 haplotype and the linear equation in Fig. 1. (IC50 nM = -73.459(mean EIS) + 69.775).

From: Polymorphism in the Plasmodium falciparum chloroquine-resistance transporter protein links verapamil enhancement of chloroquine sensitivity with the clinical efficacy of amodiaquine

EIS C5K76I
Laboratory
C34Dd2
SE Asia
C67G8Brazil Jav/IAJColombia Ecu1110
Ecuador
Cambodia Cambodia Cambodia
HAPLO. CIEI CIET SMNT CMET CMNT CMNT CIDT CTNT
72 0.04 0.04 -0.26 0.04 0.04 0.04 0.04 0.04
74 0.73 0.73 0.26 0.26 0.26 0.26 0.73 -0.18
75 -0.62 -0.62 -0.64 -0.62 -0.64 -0.64 -0.72 -0.64
76 0.73 -0.18 -0.18 -0.18 -0.18 -0.18 -0.18 -0.18
SUM 0.88 -0.03 -0.82 -0.5 -0.52 -0.52 -0.13 -0.96
MEAN 0.22 -0.0075 -0.205 -0.125 -0.13 -0.13 -0.0325 -0.24
predict IC50 DAQ (nM) 53.6 70.3 84.8 79.0 79.3 79.3 72.2 87.4
ACTUAL 54.8 69.05 86.2      
PGH-1 factor Y86---N86 Y86---N86 S. America S. America S. America Y86---N86 Y86---N86 Y86---N86
  1.4------1.0 1.4------1.0 ----------2.7 ----------2.7 ----------2.7 1.4------1.0 1.4------1.0 1.4------1.0
predict field sensitivity (IC50) nM         
IC50 DAQ (nM) 76-------54 98-------70 ---------230 ---------213 ---------213 111------79 101-----72 122-----87
  1. (To adjust for the PGH-1 factor, derived from the study of Sidhu et al, 2002 [16], multiply by 1.4 where N86Y is found in Africa and SE Asia, and 2.7 for the usual S. American pfmdr1 haplotype of S1034C, N1042D, D1246Y [27].)