TY - JOUR AU - Carter, Victoria AU - Cable, Hazel C. AU - Underhill, B. Ann AU - Williams, Jackie AU - Hurd, Hilary PY - 2003 DA - 2003/11/03 TI - Isolation of Plasmodium berghei ookinetes in culture using Nycodenz density gradient columns and magnetic isolation JO - Malaria Journal SP - 35 VL - 2 IS - 1 AB - Large scale in vitro production of the mosquito stages of malaria parasites remains elusive, with only limited success for complete sporogonic development and only one report of development through to infective sporozoites. The initial step in this process is the production, in vitro, of ookinetes from gametocytaemic blood. Methods for isolation of these ookinetes from blood cells have been described; however, in addition to yield often being low, processing time and potential for contamination by erythrocytes remain high. SN - 1475-2875 UR - https://doi.org/10.1186/1475-2875-2-35 DO - 10.1186/1475-2875-2-35 ID - Carter2003 ER -