Table 1 Comparison of ookinete yield from two purification methods. For each purification method (Nycodenz cushion v magnet), the initial density of ookinetes is expressed per ml of blood. Final yield is expressed as a percentage ± SEM of the pre-separation density, n = 4 experiments, each using the pooled blood from two infected mice. No significant difference was observed in yield between the two methods (Student T-test, p > 0.05).
Pre-separation | Nycodenz density gradient | Magnet |
|---|---|---|
Density: | Yield: | Yield: |
3.75 ± 0.48 × 107/ml | 3.8 ± 0.56 × 105/ml | 4.1 ± 0.48 × 105/ml |
Percentage: | Percentage: | Percentage: |
100% | 1.04 ± 0.17 % | 1.11 ± 0.12 % |