Table 1 Gene specific primers, cycles, product sizes and annealing temperatures.
Accession # | Cycles | Product Size (bp) | Annealing Temperature °C | Forward Primer (5'– 3') | Reverse Primer (5'-3') |
|---|---|---|---|---|---|
X63594 | 30 | 485 | 56 | CTGAAAGCTGGCTGTGATCC | TCCGTGTCATAGCTCTCCTCA |
U30186 | 25 | 416 | 56 | CTGGAAGCCTGGTATGAGGA | CGCCGTGTGGTCTCTACCT |
AI75959 | 35 | 421 | 56 | CACTGGGTAGGACACCCAAA | CCAGAACGATGGACTTTTCG |
AI237378 | 30 | 414 | 54 | GGAGCAGCAGGCTCTAGGTT | CTCGAGGAGCTAAAAGCCAAG |
M15114 | 25 | 501 | 56 | AGTGCCGTAGCTGATGGATG | CCTACCCGTGGAGGTAGGTC |
X54686 | 35 | 498 | 54 | ATCAGACACAGGCGCATCTC | TCCTCTTTAAAGGCGGAAGC |
Tubulin | 25 | 589 | 62 | CACTTCCCTCTGGCCACTTA | GTGAAAGCAGCACCTTGTGA |