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Figure 1 | Malaria Journal

Figure 1

From: Different mutation patterns of atovaquone resistance to Plasmodium falciparum in vitro and in vivo: rapid detection of codon 268 polymorphisms in the cytochrome b as potential in vivo resistance marker

Figure 1

Restriction digest method for detecting codon 268 mutations of cytochrome bc1 A: 384 bp amplification product with the primer pair CYTb3/CYTb5, digested by NsiI, K1 wild type (TAT) and AAT mutation (NGATV01) are cut (359 bp); B: 171 bp amplification product with the primer pair CYTb2/CYTb6, digested by AlwNI, only TN352 with the TCT mutation is cut to a 147 bp product; C: 174 bp amplification product with the primer pair CYTb2/CYTb7, digested by SspI, TAT wild type (K1) and TCT mutation (TN352) are digested (150 bp), while AAT mutation (NGATV01) remains uncut.

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Malaria Journal

ISSN: 1475-2875

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