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Figure 2 | Malaria Journal

Figure 2

From: Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum

Figure 2

Dot blot analysis of mouse polyclonal and monoclonal antibodies raised against the aa64-100 peptide when tested against the loop peptide and crude P. falciparum 3D7 protein extract in the presence of various reagents interfering with protein structure. The reactivity to two-fold dilutions of crude 3D7 protein extract of polyclonal and monoclonal antibodies are shown. As a positive control the reactivity to the aa64-100 peptide solution (10 μg/ml, two-fold diluted) is shown. Crude 3D7 protein extract was pre-treated before dotting as indicated: 1: untreated, 2: boiled, 3: β-ME, 4: β-ME + boiled, 5: β-ME + IAA, 6: β-ME + IAA + boiled, 7: 0.1 % SDS, 8: 0.1 % SDS + boiled, 9: 0.1 % SDS + β-ME, 10: 0.1 % SDS + β-ME +boiled, 11: 0.1 % SDS + β-ME + IAA, 12: 0.1 % SDS + β-ME + IAA + boiled, B: Buffer control.

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