Skip to main content

Table 1 Phage panning experiments ELISA plates (Dynatech Immulon I) were coated with BSA or (NPNA)3C-BSA and used in phage panning experiments. To the blocked antigen coated wells a total of 4 × 1010 pfu of the phage library in dilution buffer were added 1 × l010 pfu per well. After 4 h the wells were washed and phage eluted by applying either 0.1 M glycine-HCl pH 2.2 or a solution of the free peptide (~8 μM) (NPNA)3 dissolved in dilution buffer for 15 min at ambient temperature. An aliquot of the phage eluate was titered and the output determined.

From: Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor

Eluate after Coating antigen / Elution method (×l05 pfu)*
panning rounds BSA / acid BSA /(NPNA) 3 (NPNA) 3 C BSA /acid (NPNA) 3 C BSA /(NPNA) 3
1 2.9 (0.38) 0.82 (0.032) 3.0 (0.34) 0.51 (0.024)
2 1.4(0.03) 0.24 (0.020) 3.5 (0.24) 1.5 (0.028)
3 1.2(0.06) 0.47 (0.020) 4.3 (0.024) 12 (0.68)
4 13 (0.70) 1.0(0.032) 170 (30) 370 (20)
  1. * Figures represent the mean of the total plaque forming units eluted by either acid or excess free peptide, after repeated panning against BSA or (NPNA)3C-BSA. Values for the standard deviation are shown in brackets ().