Table 1 Phage panning experiments ELISA plates (Dynatech Immulon I) were coated with BSA or (NPNA)₃C-BSA and used in phage panning experiments. To the blocked antigen coated wells a total of 4 × 10¹⁰ pfu of the phage library in dilution buffer were added 1 × l0¹⁰ pfu per well. After 4 h the wells were washed and phage eluted by applying either 0.1 M glycine-HCl pH 2.2 or a solution of the free peptide (~8 μM) (NPNA)₃ dissolved in dilution buffer for 15 min at ambient temperature. An aliquot of the phage eluate was titered and the output determined.

From: Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor