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Figure 4 | Malaria Journal

Figure 4

From: Antibodies from malaria-exposed pregnant women recognize trypsin resistant epitopes on the surface of Plasmodium falciparum-infected erythrocytes selected for adhesion to chondroitin sulphate A

Figure 4

A modified antibody labelling procedure for FACS analysis of CSA selected parasites. In order to circumvent the non-specific labelling of FCR3CSA VSA seen when using the FITC rabbit anti-goat tertiary antibody, a biotinylated rabbit anti-human antibody in combination with FITC-conjugated streptavidin was used. Panels A and B show FCR3CSA and FCR3 infected erythrocytes respectively. In these experiments the control serum was a pool of sera from malaria naïve Danish volunteers, here shown as a solid grey histogram. The IgGmale serum pool is shown as a lightweight line and the IgGpreg serum pool as a heavyweight line.

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