Figure 1

Comparison of recombinant DBL, CIDR proteins expressed at mid-or post-log phase. Expression of GST-DBL1α and GST-CIDR1α of FCR3S1.2, GST-DBL1α and GST-DBL2β of TM284S2 was induced when the bacterial growth was at mid-log respectively post-log stages. The purified recombinant proteins were analysed in SDS-PAGE. Results presented in lane 1, 3, 5 and 7 are proteins purified from cultures where expressions was initiated at mid-log phase, while lane 2, 4, 6 and 8 show proteins after induction at post-log phase. The intact fraction of each expressed protein is marked with an arrow. Proteins were in addition verified by Western-Blot both with anti-GST-and anti-DBL1-antibodies [26].