The erythrocyte suspension (10%) was prepared from 0.8 ml of blood infected with malarial parasite (5–10% parasitaemia) as described in Materials and Methods. Three ml of 10% erythrocyte suspension was withdrawn, divided into three equal parts (1 ml each), and used in the three methods to concentrate PRBCs. After purification, the PRBC-enriched samples were suspended in 1 ml PBS. The numbers of total cells after concentration were recorded using a haemocytometer, and the number of PRBCs were determined by Field's stain. The results are representative of 3 different infected blood samples.