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Table 2 Evaluation of WBC contamination of PRBCs purified by three methods.

From: One-step concentration of malarial parasite-infected red blood cells and removal of contaminating white blood cells

Purification method

WBCs/PRBCs

WBC removal (%)

Before purification

After purification

Percoll

126.5 ± 6.3/5,000

124.4 ± 4.3/5,000

0

Nycodenz

126.5 ± 6.3/5,000

126.5 ± 7.0/5,000

0

Magnetic

126.5 ± 6.3/5,000

8.0 ± 2.1/5,000

93.7 ± 1.7

  1. WBCs before and after purification with Percoll, Nycodenz or magnetic methods were counted blindly and independently by two microscopists. Seven infected blood samples containing 5–10% parasitaemia were independently used in this study.
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Malaria Journal

ISSN: 1475-2875

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