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Table 1 Sensitivity and specificity of nested PCR to Plasmodium detection according to the different blood storage conditions.

From: Low sensitivity of nested PCR using Plasmodium DNA extracted from stained thick blood smears: an epidemiological retrospective study among subjects with low parasitaemia in an endemic area of the Brazilian Amazon region

 

Blood Storage Device

Microscopic examination

Thick Blood Smears

Filter-papers

 

Positive

Negative

Positive

Negative

Positive

40

22

45

17

Negative

22

285

120

187

Total

62

307

165

204

Sensitivity

65%

73%

Specificity

93%

61%

Observed concordance

88%

63%

Kappa coefficient (95%)

0.57

0.20